Pre-packed reverse phase columns for isolation of complex lipids synthesized from radioactive precursors.

Pre-packed reverse phase columns for isolation of complex lipids synthesized from radioactive precursors.

Pre-packed reverse phase columns (Bond Elut) were used for the separation of complex lipids, such as phosphatidylcholine, cerebrosides, sulfatides, and gangliosides, from their respective water-soluble radioactive precursors after their in vitro biosynthesis. After an incubation in vitro, the entire reaction mixture is passed through the column, where complex lipids are retained and the hydroph...

simulation of packed columns for multicomponent adsorption

the dynamics of multicomponent adsorption in a fixed bed adsorber is examined numerically. the dynamic model of adsorption in the packed column included axial dispersion and variable space velocity along the bed for the multicomponent adsorption adsorption the pore fluid and the solid phase are in equilibrium with a nonlinear isotherm. the numerical solution is based upon the modified lax-wendr...

Prediction of Mean Drop Size in Pulsed Packed Extraction Columns

Fractionation and recovery of secretions of synovial cells synthesized in culture with radioactive precursors.

The synthesis of many constituents of connective tissues, including glycosaminoglycans (Grossfeld, Meyer, Godman, and Linker, 1957), collagen (Jackson and Smith, 1957; Porter and Pappas, 1959), collagenase (Evanson, Jeffrey, and Krane, 1967) and a protein immunologically cross-reactive with chondromucoprotein (Janis, Sandson, Smith, and Hamerman, 1967), has been widely studied in cell cultures ...

A Numerical Case Study of Packed Columns

This paper presents results concerning the validation of a recently developed packing algorithm. The basic ethos of this algorithm, known as DigiPac, is to use threedimensional methods to digitise particle shapes, and to use this digital information directly in computations of how particles pack together without further conversion or the need for modelling. A variety of simulations of packed co...